TABLE 3

Oligonucleotides used in this study

PrimerSequence, 5′–3′Description of use
CDEP3165CGGAGCTCGAATTCGGATCCATTTTATCGAATATAACGTTCTCCCpET 6×His Full-length rsiV
CDEP3267TTTAAGAAGGAGATATACATATGCACCATCACCATCACCATATGGATAAGAGATTACAGCpET 6×His Full-length rsiV
CDEP3385TGTATTTTCAGGGCGCCATGATGAAATCAGAAAATGTTTCGAAGpET 6×His Full-length sipS
CDEP3054CTTTGAATTCCGGATCCCATGGAGCCCCAATGGGAAGCTGpET 6×His sipS38-184
CDEP3386TGATGGTGACGCTATGTATCCTACsipSS43A
CDEP3387ACGACATACGGCGCAAAAsipSS43A
CDEP3363TGTATTTTCAGGGCGCCATGGATTTAGTTGAAGGTTCATCTATGpET 6×His Full-length sipT
CDEP3364AGGCCTTTGAATTCCGTTATTTTGTTTGACGCATTTCGpET 6×His Full-length sipT
CDEP3365TGTATTTTCAGGGCGCCATGGTAGAAGGCGTCAGTATGpET 6×His Full-length sipV
CDEP3366AGGCCTTTGAATTCCGTTATTCGGCATCAGAAATCACpET 6×His Full-length sipV
CDEP3367TGTATTTTCAGGGCGCCATGGTTATTGAAGGATCATCAATGGCpET 6×His Full-length sipU
CDEP3368AGGCCTTTGAATTCCGTTATTTTGCCTGTCTCATCTCpET 6×His Full-length sipU
CDEP1561ATCAACCCGGACGCCGCTCAGTGGATGTCAAAGATCCCTrsiVA66W
CDEP1562AGGGATCTTTGACATCCACTGAGCGGCGTCCGGGTTGATrsiVA66W
CDEP1031AAAAAACCTCAATTTTACAGΔsigV::kan Phs-rsiV+
CDEP1012GAAGGCCATCAAGTCCAGGΔsigV::kan Phs-rsiV+
CDEP1690GGATGAATTGTTTTAGTACCTTGTTTTTTCTTCATTGCΔsigV::kan Phs-rsiV+
CDEP1691TAAGCTTAATTGTTATCCGCTAAGGAGGCTTTCTTAATGGATAAGAGATTACAGΔsigV::kan Phs-rsiV+
CDEP1692GGTACTAAAACAATTCATCCkan Phs template
CDEP1693TAAGCTTAATTGTTATCCGCkan Phs template
CDEP1701CGTCCTGGTTTTGCGCCTGGΔsipT
CDEP1711CAATTCGCCCTATAGTGAGTCGTAAAGTGACGGATCAGGAGAGCCAΔsipT
CDEP1712CCAGCTTTTGTTCCCTTTAGTGAGTGCTGGCCTAGGACAGCAGGΔsipT
CDEP1704GGTGGTTCGTCGGCTCATCGAΔsipT
CDEP1721GATGTTCACTATGTCAAACATATTATCGGCCTTCCCGGCsipSR84H
CDEP1722GCCGGGAAGGCCGATAATATGTTTGACATAGTGAACATCsipSR84H
CDEP3169TAGAATTCGAGCTCCCGGGAGTAACCTTCATGACGTTAATTGClone sipSR84H onto pMAD
CDEP3170CATGCCATGGTACCCGGGAATTGCTGTCGGAACATCClone sipSR84H onto pMAD
CDEP1955ACGACTCACTATAGGGCGAATTGTCCTTACGCGAAATACGGGC
CDEP1954CTCACTAAAGGGAACAAAAGCTGGAGTACAGTCGGCATTATCTC
CDEP3053TGTATTTTCAGGGCGCCATGGTCGTTGATGGTGACTCTATGTATCpET 6×His sipS38-184