TABLE 2

Plasmids used in this study

PlasmidFeaturesReference
pET21bampR, N-terminal 6×His expression vector, T7 promoter
pET21a 6×His-rtev-rtevampR, N-terminal 6×His-rtev expression vector, T7 promoter
pDP111aph3 (kanR) Phs66
pMADbla ermC ori pBR322 ori pE194ts63
pDP150thrC::ermR lacI+66
pAH54specR cassette67
pDR244cre ampR specR oriTS26
pAC108pET21b-rsiV (PT7-6×his-rsiV+ ampR)
pAC115pET21b-rsiVA66W (PT7-6×his-rsiVA66W ampR)
pAC112pET21a 6×His-rtev-rtev-sipS38-184 (PT7-6×his-sipS38-184 ampR)
pAC110pET21a 6×His-rtev-rtev-sipS (PT7-6×his-sipS+ ampR)
pCE601pET21a 6×His-rtev-sipT (PT7-6×his-sipT+ ampR)
pCE602pET21a 6×His-rtev-sipU (PT7-6×his-sipU+ ampR)
pCE603pET21a 6×His-rtev-sipV (PT7-6×his-sipV+ ampR)
pJLH466pMAD sipSR84H
pAC113pET21a 6×His-rtev-rtev-sipS (PT7-6×his-sipSS43A ampR)