Table 1.

Bacterial strains and plasmids used

E. coli strain or plasmidGenotypeOrganic solvent toleranceReference or source
Strains
 JA300F thr leuB6 trpC1117 thi rpsL20 hsdS n-Hexane 21
 OST3408F thr leuB6 trpC1117 thi rpsL20 hsdS marR08 Cyclohexane 1, 7
 OST3410F thr leuB6 trpC1117 thi rpsL20 hsdS Cyclohexane 5
 OST3408TcF thr leuB6 trpC1117 thi rpsL20 hsdS marR08 zde-234::Tn10 Cyclohexane 7
 JA300RF thr leuB6 trpC1117 thi rpsL20 hsdS marR08 zde-234::Tn10 CyclohexaneThis study
 JA300SF thr leuB6 trpC1117 thi rpsL20 hsdS zde-234::Tn10 n-HexaneThis study
 JA300TF thr leuB6 trpC1117 thi rpsL20 hsdS tolC DecaneThis study
 OST3408TF thr leuB6 trpC1117 thi rpsL20 hsdS marR08 tolC DecaneThis study
 CH1692 topA57 argA supD74 rpsLΔ(lac-514)tolC::Tn10 NTa 11
 MC4100F araD139 ΔlacU169 rpsL relA thi NT 23
Plasmids
 pHA1050.4-kb AflII-PvuII fragment containing the marA gene in pBluescript II 7
 pOST42BR1.9-kbSalI-BamHI fragment containing therobA gene in pBluescript II 33
 pHc3R0.4-kb HincII fragment containing thesoxS gene in pBluescript II 34
 pUXpUC19 carrying the tolC geneM. Wachi
 pMX2.5-kb EcoRI-HindIII containing the tolC gene in pMW119This study
  • a NT, not tested.