Table 2.

Effect of rpoS::cm on protease production

StrainPlasmidProtease productiona
MediumCells
92A1552 (wild type)None1,028.56 ± 58.609.76 ± 0.29
92A1552 (rpoS::cm)None9.5 ± 2.110.29 ± 0.25
pBKS7.29 ± 3.7810.60 ± 0.14
pFY127.17 ± 7.9011.60 ± 0.25
pFY75.37 ± 3.5912.56 ± 0.33
pFY8173.48 ± 18.4510.63 ± 0.36
pFY9104.83 ± 13.7711.52 ± 0.28
pFY107.95 ± 4.2310.91 ± 0.31
pWKS1305.17 ± 3.528.55 ± 0.32
  • a Means ± standard deviations from the means (n = 4); determined by measurement of fluorescence (per A 600 unit) of BODIPY FL peptides which are generated by hydrolysis of BODIPY FL casein. Supernatants of cultures grown in LB for 24 h and the cell sonicates of them were used for analysis.