Table 1.

Bacterial strains

StrainaGenotypePedigreebSource or reference(s)
BW17142DE3(lac)X74ΔphoU559::kan rpoS(Am)BD792 via BW13711 26
BW17335DE3(lac)X74Δ(pstSCAB-phoU)560::kan rpoS(Am)BD792 via BW13711 26
BW18897DE3(lac)X74 ΔphoU559 rpoS(Am) phn(EcoB)BD792 via BW18834Ilv+ with P1 on BW18833 (26)
BW21578 rrnB T14ΔlacZ WJ16 BD792 via BW21342 15
BW22831 lacI q rrnB T14 ParaB-lacZ AH31ΔaraBAD AH33 rpoS(Am)BD792 via BW22766Leu+ Ara with P1 on BW22826c (12)
BW22861 lacI q rrnB T14 PphnC-lacZ WJ19 ΔphoR574 ΔcreBCD153 Δ(pta ackA hisQ hisP)TA3516 phn(EcoB) ΔaraBAD AH33::ParaB-phoR[M1-D431]AH35 rpoS(Am)BD792 via BW22829Leu+Ara with P1 on BW22835
BW22886 rrnB T14 PrhaB-lacZ LD68ΔrhaBAD LD78 BD792 via BW22779Met+ Rha with P1 on BW22875d
BW22887 rrnB T14 PrhaB-lacZ LD68ΔrhaBAD LD78 rpoS(Am)BD792 via BW22780Met+ Rha with P1 on BW22875
BW22888 rrnB T14 PrhaS-lacZ LD69ΔrhaBAD LD78 BD792 via BW22781Met+ Rha with P1 on BW22875
BW23473Δlac-169 rpoS(Am) robA1 creC510 hsdR514 uidAMluI)::pir + endA BT333 recA1 BD792 via BW23438 12, 13
BW24249 lacI q rrnB T14 ΔlacZ WJ16ΔphoBR580 ΔcreABCD154 hsdR514 Δ(pta ackA hisQ hisP)TA3516 phn(EcoB) ΔaraBAD AH33ΔrhaBAD LD78 uidAMluI)::pir + rposS(Am) endA BT333 galU95 recA1 BD792 via BW24217Srl+ recA with P1 on BW8078 (28)
BW24486 lacI q rrnB T14 PphnC-lacZ WJ19 ΔphoBR580 ΔcreBCD153 Δ(pta ackA hisQ hisP)TA3516 phn(EcoB) ΔaraBAD AH33::ParaB-phoR[M1-D431]AH35 rpoS(Am) Δ(pstSCAB-phoU)560::ΩBD792 via BW23424Spcr and Strr with P1 on BW17636 (26)
BW24508Like BW24486 exceptatt λ::pSK58(PphoB-phoB +)BD792 via BW24486pSK58 integrant
BW24962 lacI q rrnB T14 ΔlacZ WJ16ΔphoB578 ΔcreABDC154 rpoS(Am) ΔaraBAD AH33ΔrhaBAD LD78 Δ(ackA pta)160 AH25 BD792 via BW24651Pro+ with P1 on BW23562 (13)
BW24739 lacI q rrnB T14 ΔlacZ WJ16ΔcreABDC154 rpoS(Am) ΔaraBAD AH33ΔrhaBAD LD78 Δ(ackA pta)160 AH25Δ(pstSCAB-phoR)560::ΩBD792 via BW24713Ilv+ with P1 on BW17646 (26)
BW24740 lacI q rrnB T14 ΔlacZ WJ16ΔphoB574 ΔcreABDC154 rpoS(Am) ΔaraBAD AH33ΔrhaBAD LD78 Δ(ackA pta)160 AH25Δ(pstSCAB-phoU)560::ΩBD792 via BW24715Ilv+ with P1 on BW17646 (26)
BW24741 lacI q rrnB T14 ΔlacZ WJ16ΔphoB578 ΔcreABDC154 rpoS(Am) ΔaraBAD AH33ΔrhaBAD LD78 Δ(ackA pta)160 AH25Δ(pstSCAB-phoU)560::ΩBD792 via BW24717Ilv+ with P1 on BW17646 (26)
BW24768Like BW24740 exceptatt HK022::pAH151(PrhaB-phoR +)BD792 via BW24740pAH151 integrant
BW24770Like BW24741 exceptatt λ::pAH85(PphoB-phoB +)BD792 via BW24741pAH85 integrant
BW24773Like BW24741 exceptatt λ::pAH136(PrhaB-phoB +)BD792 via BW24741pAH136 integrant
BW24774Like BW24741 exceptatt λ::pAH150(ParaB-phoB +)BD792 via BW24741pAH150 integrant
BW24775Like BW24741 exceptatt λ::pSK49(Ptac-phoB +)BD792 via BW24741pSK49 integrant
BW24777 lacI q rrnB T14 ΔlacZ WJ16ΔphoB578 ΔcreABCD154 rpoS(Am) ΔaraBAD AH33 Δ(ackA pta)160 AH25Δ(pstSCAB-phoU)560::Ω ΔrhaBAD LD78::PrhaB-phoB LD79 BD792 via BW24754Met+ Rha with P1 on BW22876
BW24803Like BW24692 exceptatt λ::pAH150(ParaB-phoB +)BD792 via BW24692pAH150 integrant
BW24858Like BW24740 exceptatt HK022::pAH152(PrhaS-phoR +)BD792 via BW24740pAH152 integrant
BW24936 lacI q rrnB T14 ΔlacZ WJ16ΔphoR574 ΔcreABCD154 rpoS(Am) ΔaraBAD AH33ΔrhaBADLD78 Δ(ackA pta)160 AH25 ΔphoU559 BD792 via BW24714Ilv+ Spcs and Strs with P1 on BW24627
BW24937 lacI q rrnB T14 ΔlacZ WJ16ΔphoB578 ΔcreABCD154 rpoS(Am) ΔaraBAD AH33ΔrhaBAD LD78 Δ(ackA pta)160 AH25 ΔphoU559 BD792 via BW24716Ilv+ Spcs and Strs with P1 on BW24627
BW24948Like BW24936 exceptatt HK022::pAH151(PrhaB-phoR +)BD792 via BW24936pAH151 integrant
BW24949Like BW24937 exceptatt λ::pAH136(PrhaB-phoB +)BD792 via BW24937pAH136 integrant
BW24950Like BW24937 exceptatt λ::pAH150(ParaB-phoB +)BD792 via BW24937pAH150 integrant
  • a All are derivatives of E. coli K-12, except that the phn(EcoB) locus is from E. coli B (31).

  • b Pedigree gives the parental strain from another laboratory and its most immediate ancestor in this laboratory. Many of our strains are descendents of E. coli K-12 strain BD792 (CGSC6159), which, like MG1655 (CGSC6300), is an unmutagenized and direct descendent of E. coli W1485 (CGSC5024) (1,2).

  • c The ara andleu-63::Tn10 markers are about 55% linked in P1kc crosses (data not shown).

  • d The relevant gene order iszii-510::Tn10 metF159 ΔrhaBAD LD78 in which Tn10 andmetF are ca. 70% linked and metF andrha are ca. 10% linked (data not shown).