Table 2.

Summary of Nuo phenotypes

Strainnuo mutation(s)Swarm plate resultaTB growth curvebGrowth on M63 + 25 mM acetatecNADH/d-NADH FeCN activitiesdEPR spectroscopy resulte
CP875None (wild type)+++++
CP366None (wild type)++NDf , g ++
AJW844 nuoB::Kmh h
AJW853 nuoB-C::CmNDND
AJW851 nuoF::miniTn10Cmh ND
CP910 nuoG::Tn10-1
CP932 nuoG::Tn10-1NDg NDND
AJW931ΔnuoG1 NDg
AJW1516ΔnuoG1 NDND
AJW1470 nuoG2 NDg NDND
AJW1517 nuoG2 NDND
AJW845 nuoH::Kmh h
AJW846 nuoI::Kmh h
AJW852 nuoM::miniTn10CmNDND
AJW847 nuoN::Kmh +h
AJW932CP366nuoΩ(pHF17)++NDg NDND
AJW1459AJW931nuoΩ(pAJW105ΔPstI)++NDg NDND
AJW1472AJW1470nuoΩ(pAJW105ΔPstI)++NDg NDND
AJW1582ΔnuoG1 nuoH::KmNDND
AJW1583ΔnuoG1 nuoI::KmNDND
AJW1584 nuoG2 nuoH::KmNDND
  • a +, presence of an inner, aspartate ring; −, absence of the inner ring.

  • b +, no growth defect; −, growth defect.

  • c +, good growth (≥1.0-mm colonies); −, poor growth (≤0.5-mm colonies).

  • d +, wild-type levels of both activities in membrane fractions; −, less than 20% of wild-type activities.

  • e +, at least three FeS centers detected; −, no detectable FeS centers.

  • f ND, not done for this study.

  • g The strain background was Aceprior to introduction of the nuo mutation(s).

  • h The mutation was analyzed in a different, but related, background.