Table 3.

Oligonucleotide primers

NameDescriptionSequencea
P109 phoR N terminus5′-GGAATTCGTCATA TGCTGGAACGGC-3′
P110 phoR C terminus5′-CGGGATCCGCTGAGCTCAGTCGCTGTTTTTGGC-3′
P119 PrhaSB upstream5′-CCGCTCGAGGCCATGG TGGCCTCCTGATGTCG-3′
P120 PrhaSB downstream5′-CCGGTCGACATATG GTGATCCTGCTGAATTTC-3′
P121 rhaD′ upstream5′-CGGGATCCGCATGC GTCGACAGCGACGG-3′
P122 rhaD′ downstream5′-CGTACGTACG CAGCGCCAGCGCAC-3′
P125 ParaB downstream5′-GCGCGCTGCA GGTATGGAGAAACAGTAGAG-3′
P126 ParaB upstream5′-GGTCCTCGA GCGAATGGTGAGATTG-3′
P127araD′ upstream5′-GACACGTCGACGCATGC AGATCTCAAACGCCAG-3′
P128araD′ downstream5′-CGTACGTA CGACCTCTTCCAGCAC-3′
  • a Underlined bases are complementary to the template(s). Those in boldface indicate restriction enzyme sites.