Table 1.

Forward and reverse primers used in construction of probes

GeneForward and reverse primerPCR product size (bp)ProbeReference
sfaA1 5′ CGG TGT GCG TAG TTC AAT 3′ 32
sfaA2 5′ CAC CCG CAT GGA TAA AAA 3′641sfa1 32
sfaG1 5′ CAT TAA CTC CCG AAA CTT 3′ 29
sfaH2 5′ CAT TAC CGC CAC AAC TGC 3′1,862sfa2 29
papA1 5′ TTA AAG GTA ATC GGG TCA T 3′ 22
papA2 5′ GGA ATC AGA GAA AAG GTT 3′856pap1 22
papA1 5′ TTA AAG GTA ATC GGG TCA T 3′ 22
papC2 5′ CGC TTC AGG TCA ACA GAG G 3′3,512pap2 22
papH1 5′ AAT ACT GGG GAG AAG AGC AC 3′ 22
papF2 5′ ATT ACG AAA GGG CAC TGA AG 3′6,086pap3 22
kpsE1 5′ AAA AGA CCC GTG TAG AAG C 3′ 26
kpsC2 5′ CAA AAG GTC AGA GCC AAG T 3′3,852kps1 26
kpsD2 5′ CGTTACGGG AAT GCT GCT T 3′ 26
kpsM1 5′ GTC CAG AAA GTC ACC GTA GA 3′ X53819 a
kpsT2 5′ CAA TCG CCA CAT CAC AAA AC 3′1,335kps2 X53819 a
hly1 5′ TCA GTC CTC TTT CTT TCC T 3′ U12572 a
hly2 5′ CTC TGG CAA CGG TCT CTC C 3′2,899hly1 U12572 a
hlyA1 5′ GAC AAA GCA CGA AAG ATG 3′ 8
hlyA2 5′ CAA CTG CAA TAA AGA AGC 3′2,930hly2 8
  • a GenBank accession number.