Table 1.

Styrene pathway genes, their products, and identities with other proteinsa

Gene% G+C contentGene product (aa/kDa)Similar polypeptide (aa)% IdentityOrganismAccession no.
paaK63.6437/49.1Pcl (439)86.3Pseudomonas putida UZ71175
o437 (437)67.3Escherichia coli K-12AE000236
PaaK (437)67.3E. coli WX97452
styS56.8982/108.8TutC (979)46.5Thauera sp. strain T1U57900
TodS (978)41.2P. putida F1U72354
NodV (889)24.5Bradyrhizobium japonicumP15939
BpdS (1576)18.3Rhodococcus sp. strain M5U85412
FixL (505)18.3Rhizobium melilotiP10955
styR52.9207/23.3TutB (218)51.4Thauera sp. strain T1U57900
NodW (227)45.8B. japonicumP15940
TodT (227)42.4P. putida F1U72354
FixJ (204)41.1R. melilotiP23221
BpdT (209)26.7Rhodococcus sp. strain M5U85412
styA58.8415/46.6StyA (415)94.5P. fluorescens STZ92524
PobA (394)22.3P. aeruginosaX68438
NahG (434)20.6P. putida PpG7P23262
MhpA (554)18.1E. coli K-12Y09555
styB57.3170/18.4StyB (170)94.1P. fluorescens STZ92524
SnaC (176)29.2Streptomyces pristinaespiralisP54994
ActRV (177)24.1S. coelicolorA3(2)X63449
HpaH (170)22.3Klebsiella pneumoniae M5a1L41068
HpaC (170)21.2E. coli WZ29081
NmoB (322)18.6Chelatobacter heintziiL49438
NtaB (335)17.9C. heintziiU39411
styC55.9169/18.0StyC (169)92.3P. fluorescens STZ92524
styD59.4502/53.5StyD (502)97.8P. fluorescens STZ92524
CymC (494)45.9P. putida F1U24215
ALDH Mt (498)45.5Leishmania tarentolaeS43184
PadA (499)45.3E. coli WX97453
FeaB (500)45.1E. coli K-12X99402
ALDH Ct (501)44.6Ovis ariesP51977
porA58.8433/45.9CumH (457)51.0P. fluorescens IPO1D83955
XylN (464)47.6P. putida mt-2D63341
TodX (453)41.8P. putida F1P10384
CymD (460)40.3P. putida F1U24215
  • ↵a Sequences included in this analysis: Pcl, phenylacetyl-coenzyme A ligase of the phenylacetate catabolic pathway; PaaK, phenylacetyl-coenzyme A ligase of the phenylacetate catabolic pathway (12); o437, protein of unknown function; TutC and TutB, sensor histidine kinase and response regulator of the toluene anaerobic catabolic pathway, respectively; TodS and TodT, sensor histidine kinase and response regulator of the toluene aerobic catabolic pathway, respectively; BpdS and BpdT, sensor histidine kinase and response regulator of the biphenyl/polychlorobiphenyl catabolic pathway, respectively; NodV and NodW, sensor histidine kinase and response regulator of the nodulation genes, respectively; FixL and FixJ, sensor histidine kinase and response regulator of the nitrogen fixation genes, respectively; StyA and StyB, styrene monooxygenase components; PobA, 4-hydroxybenzoate 3-hydroxylase; NahG, salicylate hydroxylase; MhpA, 3-(3-hydroxyphenyl) propionate hydroxylase; SnaC, NADH:FMN oxidoreductase of the pristinamycin IIA synthase; ActRV, NADH:FMN oxidoreductase of actinorhodin synthesis, dimerase; HpaH and HpaC, coupling proteins of 4-hydroxyphenylacetate 3-hydroxylase of the 4-hydroxyphenylacetate catabolic pathway; NmoB and NtaB, NADH:FMN oxidoreductase of the nitrilotriacetate catabolic pathway; StyC, epoxystyrene isomerase; StyD, phenylacetaldehyde dehydrogenase of the styrene catabolic pathway; CymC,p-cumic aldehyde dehydrogenase; ALDH Mt, mitochondrial aldehyde dehydrogenase (NAD+) precursor; PadA and FeaB, phenylacetaldehyde dehydrogenases of the 2-phenylethylamine catabolic pathway; ALDH Ct, cytosolic aldehyde dehydrogenase; CumH, XylN, TodX, and CymD, proposed outer membrane proteins and putative transporters of cumene, toluene/xylene, and p-cymene, respectively.