Table 1.

Styrene pathway genes, their products, and identities with other proteinsa

Gene% G+C contentGene product (aa/kDa)Similar polypeptide (aa)% IdentityOrganismAccession no.
paaK 63.6437/49.1Pcl (439)86.3 Pseudomonas putida U Z71175
o437 (437)67.3 Escherichia coli K-12 AE000236
PaaK (437)67.3 E. coli W X97452
styS 56.8982/108.8TutC (979)46.5 Thauera sp. strain T1 U57900
TodS (978)41.2 P. putida F1 U72354
NodV (889)24.5 Bradyrhizobium japonicum P15939
BpdS (1576)18.3 Rhodococcus sp. strain M5 U85412
FixL (505)18.3 Rhizobium meliloti P10955
styR 52.9207/23.3TutB (218)51.4 Thauera sp. strain T1 U57900
NodW (227)45.8 B. japonicum P15940
TodT (227)42.4 P. putida F1 U72354
FixJ (204)41.1 R. meliloti P23221
BpdT (209)26.7 Rhodococcus sp. strain M5 U85412
styA 58.8415/46.6StyA (415)94.5 P. fluorescens ST Z92524
PobA (394)22.3 P. aeruginosa X68438
NahG (434)20.6 P. putida PpG7 P23262
MhpA (554)18.1 E. coli K-12 Y09555
styB 57.3170/18.4StyB (170)94.1 P. fluorescens ST Z92524
SnaC (176)29.2 Streptomyces pristinaespiralis P54994
ActRV (177)24.1 S. coelicolorA3(2) X63449
HpaH (170)22.3 Klebsiella pneumoniae M5a1 L41068
HpaC (170)21.2 E. coli W Z29081
NmoB (322)18.6 Chelatobacter heintzii L49438
NtaB (335)17.9 C. heintzii U39411
styC 55.9169/18.0StyC (169)92.3 P. fluorescens ST Z92524
styD 59.4502/53.5StyD (502)97.8 P. fluorescens ST Z92524
CymC (494)45.9 P. putida F1 U24215
ALDH Mt (498)45.5 Leishmania tarentolae S43184
PadA (499)45.3 E. coli W X97453
FeaB (500)45.1 E. coli K-12 X99402
ALDH Ct (501)44.6 Ovis aries P51977
porA 58.8433/45.9CumH (457)51.0 P. fluorescens IPO1 D83955
XylN (464)47.6 P. putida mt-2 D63341
TodX (453)41.8 P. putida F1 P10384
CymD (460)40.3 P. putida F1 U24215
  • a Sequences included in this analysis: Pcl, phenylacetyl-coenzyme A ligase of the phenylacetate catabolic pathway; PaaK, phenylacetyl-coenzyme A ligase of the phenylacetate catabolic pathway (12); o437, protein of unknown function; TutC and TutB, sensor histidine kinase and response regulator of the toluene anaerobic catabolic pathway, respectively; TodS and TodT, sensor histidine kinase and response regulator of the toluene aerobic catabolic pathway, respectively; BpdS and BpdT, sensor histidine kinase and response regulator of the biphenyl/polychlorobiphenyl catabolic pathway, respectively; NodV and NodW, sensor histidine kinase and response regulator of the nodulation genes, respectively; FixL and FixJ, sensor histidine kinase and response regulator of the nitrogen fixation genes, respectively; StyA and StyB, styrene monooxygenase components; PobA, 4-hydroxybenzoate 3-hydroxylase; NahG, salicylate hydroxylase; MhpA, 3-(3-hydroxyphenyl) propionate hydroxylase; SnaC, NADH:FMN oxidoreductase of the pristinamycin IIA synthase; ActRV, NADH:FMN oxidoreductase of actinorhodin synthesis, dimerase; HpaH and HpaC, coupling proteins of 4-hydroxyphenylacetate 3-hydroxylase of the 4-hydroxyphenylacetate catabolic pathway; NmoB and NtaB, NADH:FMN oxidoreductase of the nitrilotriacetate catabolic pathway; StyC, epoxystyrene isomerase; StyD, phenylacetaldehyde dehydrogenase of the styrene catabolic pathway; CymC,p-cumic aldehyde dehydrogenase; ALDH Mt, mitochondrial aldehyde dehydrogenase (NAD+) precursor; PadA and FeaB, phenylacetaldehyde dehydrogenases of the 2-phenylethylamine catabolic pathway; ALDH Ct, cytosolic aldehyde dehydrogenase; CumH, XylN, TodX, and CymD, proposed outer membrane proteins and putative transporters of cumene, toluene/xylene, and p-cymene, respectively.