Table 2.

Oligonucleotides used

NameSequenceDescription
Universal-PCRGTAAAACGACGGCCAGTGAGCGCGCBinds the KpnI side of the pCR-Script multiple cloning site (Stratagene)
Reverse-PCRGGAAACAGCTATGACCATGATTACGBinds theSacI side of the pCR-Script multiple cloning site (Stratagene)
Tn3-blaGACAGATCGCTGAGATAGGTGCCTCBinds the end of mTn3 (oriented outward) within the β-lactamase gene
BA134TGCGTTTTCAGAGGATGGAGACBinds nucleotides 501 to 522 of E. coli sdiA (accession no. X03691 )
BA135AGGCAACCTGGGTCTTATTTGGBinds nucleotides 1154 to 1133 of E. coli sdiA (accession no. X03691 )
BA174CAACAAGAAGAACGTTGATCAAAGGBinds nucleotides 1970 to 1946 of the sequence reported here (accession no. U88651 )
BA176CGAGTGAAATACGCGTAATAAACBinds nucleotides 1678 to 1656 of the sequence reported here (accession no. U88651 )
BA178AATGATTATCAATATCAAAGGCGTGBinds nucleotides 437 to 461 of the sequence reported here (accession no. U88651 )
MudOutCCGAATAATCCAATGTCCTCCCGGTBinds nucleotides 54 to 30 of the Mu left end (accession no. M64097 )
MudTaqAGTGCGCAATAACTTGCTCTCGTTCBinds nucleotides 701 to 725 of the Mu left end (accession no. M64097 )
MudAluCGAAAAACAAAAACACTGCAAATCATTTCAATAACBinds nucleotides 167 to 201 of the Mu left end (accession no. M64097 )