Table 4.

Regulation of ParaB -,PrhaB -, andPrhaS-lacZ fusions

StrainRelevant genotypeaCarbon sourceβ-Galactosidase sp act onb:Fold induction
−Ind+Ind
BW22831 ParaB-lacZ rpoS(Am) d-Glucose6.6 ± 0.11,270 ± 114190
d-Fructose6.7 ± 0.63,861 ± 313580
Glycerol6.5 ± 0.43,853 ± 119590
BW22887 PrhaB-lacZ rpoS(Am) d-Glucose0.5 ± 0.0743 ± 441,600
d-Fructose0.5 ± 0.03,300 ± 676,900
Glycerol0.5 ± 0.03,689 ± 727,800
BW22886 PrhaB-lacZ rpoS + d-Glucose0.4 ± 0.0185 ± 10430
d-Fructose0.4 ± 0.01,158 ± 332,700
Glycerol0.4 ± 0.02,209 ± 505,800
BW22888 PrhaS-lacZ rpoS + d-Glucose0.4 ± 0.056 ± 2130
d-Fructose0.5 ± 0.1253 ± 5490
Glycerol0.8 ± 0.1619 ± 21810
  • a All strains are also ΔaraBAD or ΔrhaBAD, as appropriate; complete genotypes are given in Table 1.

  • b Cells were assayed after 18 h of growth in 0.06% glucose–, 0.06% fructose–, or 0.1% glycerol–MOPS–2 mM Pi without (−Ind) or with (+Ind) the inducer arabinose or rhamnose. Values are nanomoles of o-nitrophenol made per unit of cell culture optical density at 420 nm (means ± standard deviations). Strains were grown and assayed in triplicate.