Table 1.

Bacterial strains and plasmidsa

Strain or plasmidRelevant characteristicsSource or reference
Y. pestis
 KIM6+Pgm+Ybt+ Yfe+Lcr 68
 KIM6Pgmpgm) YbtYfe+ Lcr 32, 68
 KIM6-2030Pgm Fur(fur::kan-9) YbtYfe+ LcrKmr 73
Y. enterocolitica WASerotype O:8, Ybt+Yfu+ Psts Lcr+ 32,82
Y. pseudotuberculosisPB1/+Serotype 1, Psts Lcr+ 32,82
E. coli
 CC118Strain for detecting PhoA+ fusion proteins, PhoA 53
 DH5αCloning strain, Yfe PhoA 5
 HB101K-12 × B hybrid strain, Ent+Yfe Smr 5
 LE392Strain for propagating λTnphoA 5
 SAB11Ent derivative of HB101 6
Plasmids
 pACYC1844.2-kb cloning vector, CmrTcr 5
 pBluescript II KS+ 3.0-kb cloning vector, Apr Stratageneb
 pBR3224.4-kb cloning vector, Apr Tcr 5
 pUC192.7-kb cloning vector, Apr 65
 pEUPP115.3-kb pEU730-derived reporter plasmid containing the psn promoter region upstream of a promoterless lacZ gene, Spr 29
 pSC27.1Reporter plasmid containing an iron- and Fur-regulated lacZgene 17
 pYHE3pHC79-derived cosmid clone containing an ∼23-kb fragment from Y. pestis KIM6+ genomic DNA, Apr This study
 pYFE112.8-kbBamHI fragment from pYHE3 cloned into pUC19, Yfe+ Apr This study
 pYFE1.112.8-kbBamHI fragment from pYHE3 cloned into pACYC184, Yfe+ Cmr This study
 pYFE1.2pYFE1.1 with BamHI fragment in the opposite orientation, Yfe+ Cmr This study
 pYFE25.2-kbClaI/BamHI fragment of pYFE1 inserted into pBR322, Yfe Apr This study
 pYFE37.7 kb BamHI/ClaI fragment of pYFE1 cloned into pBR322, Yfe+ Apr This study
 pYFE3.17.7-kb BamHI/ClaI fragment of pYFE3 cloned into pBluescript, Yfe+Apr This study
 pYFE45.3-kbBamHI/HindIII fragment of pYFE1 inserted in pBR322, Yfe Apr This study
 pYFE63.6-kb PvuI/PstI fragment of pYFE1 cloned into pBR322, Yfe Tcr This study
 pYFE89.7-kb NcoI fragment of pYFE1 cloned into pACYC184, Yfe+ Tcr This study
 pYFE96.1-kb PstI fragment of pYFE1 inserted into pBR322, Yfe Tcr This study
 pYFE10pYFE1.1 lacking internal 5.3-kb SphI fragment, Yfe+ Cmr This study
 pYFE11pYFE3 with a cat cassette inserted at theHindIII site, Yfe+/− AprCmr This study
 pYFE12pYFE10 with akan cassette inserted at the PstI site, Yfe Kmr Cmr This study
 pYFE13pYFE10 with a kan cassette inserted at theXhoI site, Yfe KmrCmr This study
 pYFE15pYFE1 with 4.5-kbXhoI fragment deletion, YfeApr This study
 pYFE214.2-kbEcoRI/PstI fragment of pYFE1 cloned into pBluescript, YfeyfeAB) Apr This study
 pYFE30∼1.8-kbEcoRI fragment deletion of pYFE3.1, YfeyfeAB) Apr This study
 pYFE34 to -46TnphoA insertion mutants of pYFE1.1 (Fig. 1B), Cmr Kmr This study
 pYFE4714.2-kbSalI fragment deletion of pYFE34, Yfe, PhoA+ reporter for Y. pestis KIM6-2030, Cmr Kmr This study
  • a Y. pestis KIM6+ possesses an intact 102-kb pgm locus containing the genes for hemin storage (hms), psn, ybtA, and the Ybt biosynthetic genes irp1, irp2, ybtT, and ybtE. Y. pestis KIM6 is an isolate of KIM6+ with the 102-kb region deleted. Yersinia strains producing the siderophore yersiniabactin have been designated Ybt+, while those defective in yersiniabactin secretion are Ybt. The Yfe+ designation indicates the presence of operons yfeABCD and yfeE. Mutations in either yfe operon are depicted as Yfe. Lcr+ and Lcr refer to the presence or absence, respectively, of the low-calcium response phenotype conferred by the 70- to 75-kb virulence plasmid. Ent+ and Ent indicate production or lack of the siderophore enterobactin, respectively. E. colistrains which fail to express alkaline phosphatase are designated PhoA. Apr, Cmr, Kmr, Smr, Spr, and Tcr, resistance to ampicillin, chloramphenicol, kanamycin, streptomycin, spectinomycin, and tetracycline, respectively.

  • b Stratagene Cloning Systems, La Jolla, Calif.