Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)Source or reference
S. paucimobilisstrains
 UT26Growth on γ-HCH; Nalr8
 UT102NalrKmr18
 UT103NalrKmr18
 UT116NalrKmr18
P. putida strain
 PpY101metNalr11
E. coli strains
 HB101FhsdS recA ara proA lacY galK rpsL xyl mtl supE12
 MV1190Δlac-proAB thi supE Δsrl-recA306::Tn10 F′ traD36 proAB lacIqZΔM1517
Plasmids
 pAQNpMB9 replicon;lacIqaqnaApr23
 pKM1pUC18 carrying 3-kb SacI-HindIII fragment; direction oflinD is identical to that of lac promoter of pUC18This study
 pKM1RpUC18 carrying same fragment as pKM1 with opposite directionThis study
 pKM2pUC18 carrying 1.2-kb EcoRI-MluI fragment of pKM170This study
 pKM2RpUC18 carrying same fragment as pKM2 with opposite directionThis study
 pKM170pUC18 carrying 2.0-kb fragment of pKM1; deletion plasmid with deletion about 900 bp from 5′ end of fragment inserted in pKM1This study
 pKM9372pUC18 carrying 8-kb EcoRI fragment of pMM9372This study
 pKS13RK2 replicon; cos Mob+Tcr11
 pKSM937pKS13 with 20 kb of UT26 DNA containing linDThis study
 pMFY42RSF1010 replicon; KmrTcr4
 pMM9372pMFY42 carrying 8-kb EcoRI fragment of pKSM937This study
 pMYLB1pAQN carrying linB in place ofaqna16
 pMYLD2pAQN carrying linD in place of aqnaThis study
 pRK2013ColE1::RK2 Tra+Kmr3
 pUC18pMB9 replicon of pKM9372; Apr26
  • a aqn, aqualysin I gene of Thermus aquaticus.