Table 1.

Bacterial strains used

StrainGenotypeReference or source
E. coliK-12
 DH5α endA1hsdR17(r K m K )supE44thi-1 recA1 gyrA relA1Δ(lacZYA-argF)U169 deoR80 dlacΔ(lacZM15)] 15
 S17-1 λpir prp thi recA hsdR; chromosomal RP4-2 (Tn1::ISR1 tet::Mu Km::Tn7); λpir 33
 AB2847 aroB malT tsx thi 17
 H5058 aroB malT tsx thi cir fiu fepA K. Hantke
S. enterica serotype Typhimurium
 14028Wild type (isolated from bovine septicemia)American Type Culture Collection
 CL150914028aroA::Tn10 8
 AR125814028entB::MudJ 38
 IR71514028 Nalr 35
 AJB2014028 Nalr iroBC::Kmr 5
 AIR4914028 Nalr iroBC::Kmr aroA::Tn10 This study
 AJB5214028 Nalr iroN::pGP704This study
 AJB6414028 Nalr iroN::pGP704aroA::Tn10 This study
S. enterica serotype Typhi
 AJB70Wild type (clinical isolate, San Diego, Calif.) 5
 TY21a galE viaB rpoS 14
 AJB22TY21a Nalr 5
 AJB54TY21a Nalr iroN::pGP704This study