Table 3.

Complementation of SVM167

StrainCHOHEp-2
% Invasiona% of wild-type level% Invasion% of wild-type level
14028s (wild type)4.8 ± 0.31005.3 ± 0.3100
14028s (pWKS30)2.3 ± 0.01005.7 ± 0.8100
14028s (pHH20)2.2 ± 0.051003.3 ± 0.2100
14028s (pHH26)4.5 ± 0.151007.8 ± 0.1100
SVM167 (sigE::Tn10dTc)0.3 ± 0.076.30.53 ± 0.0610
SVM167 (pWKS30)0.2 ± 0.028.70.4 ± 0.077
SVM167 (pHH20)0.8 ± 0.05361.6 ± 0.349
SVM167 (pHH26)1.5 ± 0.2332.3 ± 0.0235
  • a Percentage of intracellular bacteria ± range after allowing bacteria to invade during a 1-h incubation; duplicate assays were performed. Values are representative of several assays done in this study.