Table 2.

Characterization of novel invasion mutants

Strain% Invasiona (% of wild-type level)Map positionHomology to region of insertionc
CHOHEp-2
14028s (wild type)2.2 ± 0.2 (100)5.3 ± 0.3 (100)
SVM1670.2 ± 0.2 (9)0.4 ± 0.02 (7.5)∼25.5b Shigella ipgDE(F)
SVM1680.5 ± 0.6 (22)1.8 ± 0.07 (34)∼78d S. enteritidis IS1351 ORFB; Y. enterocoliticahigh-pathogenicity island ORFB
  • a Percentage of intracellular bacteria ± range after bacteria were allowed to invade during a 1-h incubation; duplicate assays performed. Values are representative of several assays done in this study.

  • b Map (centisome) position of the Tn10dTc insertions was determined by MudP22 transduction mapping (SVM167). The MudP22 element in strain TT15240 (putA1019::MudP) cotransduced with the Tn10dTc insertion in SVM167.

  • c Homologies are at the amino acid sequence level.

  • d The insertion in SVM168 was mapped by using Hfr strains with origins of transfer at 76 and 78 min of the LT2 chromosome.