Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or descriptionReference or source
E. coli
 DH5αFΦ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK mK +) supE44 thi-1 gyrA69 6
 RR1F mcrB mrr hsdS20 (rB mB ) ara14 proA2 lacY1 leu galK2 rpsL20 Smr xyl5 mtl1 supE44 46
B. subtilis
 IS58 trpC2 lys-3 48
 BGH1 trpC2 lys-3 sigB::Δ(HindIII-EcoRV)::cat 32
 BSA115 trpC2 rsbU::kanPBΔ28::PSPAC rsbW313pTet-I SPβctc::lacZ 54
 BIG1 trpC2 lys-3 trxA::pHV501pHVES1→IS58
 BD224 trpC2 recE4 10
Plasmids
 pBluescript II SK(+/−)  or KS(+/−)Cloning vector, Apr Stratagene
 pWH703Promoter probe vector, XylE+ CmrKmr 52
 pWH262pWH703 containing a 120-bp SauIIIA fragment carrying the 5′ region of trxA This study
 pHV501Integrative plasmid, Apr Emr 51
 pHVES1pHV501 containing a 240-bp trxA fragment generated by PCRThis study
 pKSES1pBluescript II KS containing a 459-bp fragment generated by PCRThis study
 pSKES1pBluescript II SK containing a 720-bp ClaI fragment generated by inverse PCRThis study
 pSKES2pBluescript II SK containing a 950-bp EcoRI fragment generated by inverse PCRThis study
 pSKES3pBluescript II SK containing a 1.4-kb StyI fragment generated by inverse PCRThis study
 pSKES4pBluescript II SK containing a 1.6-kb PvuII fragment generated by inverse PCRThis study