Table 1.

Comparison of putative DinR boxes from the dingenes of B. subtilis and the genes of several gram-positive bacteria

OrganismGeneaDinR binding sitebAccession no.
Bacillus subtilis recA(−51) CGAATATGCGTTCG* U56238
dinA CGAACTTTAGTTCG* M64048
dinB AGAACTCATGTTCG* M64049
dinC(−24) CGAACGTATGTTTG* M64050
dinC(−53) AGAACAAGTGTTCG*
dinR(−39) CGAACCTATGTTTG* M64684
dinR(−67) CGAACAAACGTTTC*
dinR(−104) GGAATGTTTGTTCG*
Bacteroides fragilis recA(−20) CGAATTAAACTTTG M63029
recA(−107) CGAACGGATCATCG
Clostridium perfringens recA AGAACTTATGTTCG U61497
Corynebacterium glutamicum recA CGTAGGAATTTTCG U14965
Corynebacterium pseudotuberculosis recA AGAATGGTCGTTAG U30387
Deinococcus radiodurans recA CGATCCTGCGTAAG U01876
Mycobacterium leprae recA CGAACAGATGTTCG X73822
Mycobacterium smegmatis recA CGAACAGGTGTTCG* X99208
Mycobacterium tuberculosis recA CGAACAGGTGTTCG* X58485
lexA CGAACACATGTTTG* X91407
Staphylococcus aureus recA CGAACAAATATTCG L25893
Streptococcus mutans recA CGAACATGCCCTTG M81468
Streptomyces lividans recA CGAACATCCATTCT X76076
Thermotoga maritima recA CGAATGTCAGTTTG L23425
  • a The location of the DinR box with respect to identified promoter elements is noted in parentheses.

  • b The consensus DinR binding site,C GAACRNRYGTTC G, is defined as occurring in at least 70% of the sequences analyzed. With standard nomenclature, R = G or A and Y = C or T. The most-5′ and most-3′ nucleotides of the DinR box are in bold-faced type, while those of the previously identified Cheo box are underlined. Sequences with an asterisk denote sites that have been experimentally shown to physically bind DinR (or a DinR-like protein): B. subtilis recA (16, 27), dinA (Fig. 2),dinB (Fig. 2; [16]), dinC (Fig.3; [16]), and dinR (Fig. 4; [5]); M. smegmatis recA (4,19); M. tuberculosis recA (19) and lexA (17). The consensus sequence of the derived DinR box is 5′-CGAACRNRYGTTCG-3′.