Table 3.

Substrate specificities of 4NBZDH activitiesa

SubstrateValue for source of cell extract
TW3 grown on 4-nitrotolueneTW3 grown on tolueneE. coliBL21(DE3) expressing ntnC
4-Nitrobenzaldehyde100100100
3-Nitrobenzaldehyde121126120
4-Methylbenzaldehyde282725
3-Methylbenzaldehyde222220
Benzaldehyde404337
  • a Activities are expressed as percentages relative to that observed with 4-nitrobenzaldehyde, each value being the mean from two different cultures. No individual value was more than ±3% from the mean.