Table 1.

Bacterial strains, plasmids, and phages

Strain, plasmid, or phageGenotypeSource
S. peucetius
 ATCC 29050Wild typeATCCa
 WMH1650dnrX aphII, obtained after infection with phWHM281This work
 WMH1655dpsY aphII, obtained after infection with phWHM283This work
S. lividans
 TK24SLP1 SLP2, streptomycin resistant19
 WMH1594SLP1SLP2, streptomycin resistant, φC31 lysogen23
Plasmids
 pFDNeoSpUC18 containing theaphII gene7
 pWHM249XhoI site eliminated from theaphII gene of pFDNeoS23
 pWHM335Cosmid clone containing a fragment of the DNR cluster40
 pWHM2793.3-kbBamHI fragment cloned from pWHM335 in pUC18This work
 pWHM2801.0-kb SalI fragment with theaphII gene cloned blunt ended from pWHM249 into the filled-in NcoI site of pWHM279This work
 pWHM2821.0-kb SalI fragment with theaphII gene cloned blunt ended from pWHM249 into the filled-in EcoNI site of pWHM279This work
 pWHM3461.6-kb SstI-SphI fragment cloned from pWHM279 into pSP72This work
 pWHM3471.6-kbEcoRI-HindIII fragment cloned from pWHM346 into pWHM3This work
 pWHM2263.3-kb BamHI fragment cloned from pWHM279 into pWHM3This work
Phages
 KC515c+ attP, thiostrepton resistant, viomycin resistant5
 phWHM281c+ attP, viomycin resistant; 4.3-kb BamHI fragment cloned from pWHM280 into KC515 between the BamHI and BglII sitesThis work
 phWHM283c+ attP, viomycin resistant; 4.3-kb BamHI fragment cloned from pWHM282 into KC515 between the BamHI and BglII sitesThis work
  • a ATCC, American Type Culture Collection.