Table 1.

E. coli K-12 strains and plasmids used

Strain or plasmidDescriptionSource or reference
Strains
 β63MG1655 malA-kan mutTP. Marlière
 CT110CSH57thiA(pULB110)A. Toussaint
 HB101FΔ(gpt-proA)62 leuB6 supE44 ara-14 galK2 lacYI proA2 rpsL20 xyl-5 mtl-1 recA13 Δ(mrcC-mrr)Laboratory collection
 MC4100araD139Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsRLaboratory collection
 MG1655λF prototrophLaboratory collection
 MH225MC4100 Φ(ompC-lacZ+)10–2510
 MH513MC4100 Φ(ompF-lacZ+)16–1310
 PHL628MG1655 malA-kan ompR234aThis study
 PHL644MC4100 malA-kan ompR234This study
 PHL645MC4100 malA-kanThis study
 PHL690MG1655 malA-kanThis study
 PHL691HB101(pR′7)This study
 PHL744MC4100malT54::Tn10 ompR234This study
 PHL745MC4100 malT54::Tn10 ompR234 csgA::kanThis study
 PHL804MC4100csgA::kanThis study
 PHL806PHL804(pCSG4)This study
 PHL839MH513malA-kan ompR234This study
 PHL840MH225malA-kan ompR234This study
 PHL841MH513malA-kanThis study
 PHL842MH225malA-kanThis study
 PHL874PHL804(pOV874)This study
 PHL875PHL745(pCSG4)This study
 PHL876PHL745(pOV874)This study
 PHL893TK821csgA::kan(pCSG4)This study
 PHL894TK821csgA::kan(pOV874)This study
 RL101β63 ompR234This study
 RL102 to -111β63 adr-102 to adr-111This study
 TK821MC4100ompR331::Tn1010
 TST3FaraD139Δ(argF-lac)169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR malT54::Tn10CGSC 6139
 YMel-1YMelcsgA::kan21
Plasmids
 pAT003pBR322 with a 7.8-kbBamHI fragment harboring the ompBoperon19
 pBR325AprTcr CmrLaboratory collection
 pCSG4pUC19 with a 3.5-kb HindIII fragment containing the csgBA operon21
 pOV874pCSG4 carrying a uidA-kan fusion gene inserted at the unique ClaI site of the csgAgeneThis study
 pN496pBluescript Cmrcontaining a uidA-kan gene fusion13
 pOV711pBR325 with a 7.8-kb BamHI fragment harboring the ompR234 mutationThis study
 pOV737pBR325 with a 1.285-kb EcoRI-SmaI fragment harboring the ompR234 mutationThis study
 pR′7pULB110 derivative harboring themalA::kan marker and theompR234 mutation of PHL628This study
 pULB110RP4::Mu3A, Apr TcrKmsA. Toussaint
  • a ompR234 is equivalent toadr-101 (see text).