Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype and phenotypeSource or reference
E. coli strains
 SM10λpirthi recA thr leu tonA lacY supE RP4-2-Tc::Mu λ::pirLaboratory strain
 DH5αλpirFΔ(lacZYA-argF)U169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 λ::pir10, 12
 AC-E298Sm10λpir (pAC298)This work
 AC-E303Sm10λpir (pAC303)This work
 AC-E355Sm10λpir (pSL116)This work
V. cholerae strains
 AC-V51C6709-1, El Tor biotype, Smr19
 AC-V66AC-V51lacZ::res1-tet-res1, Tcr5
 AC-V186AC-V51iviVII::pGP7045
 AC-V232AC-V66iviVII::pIVET55
 AC-V296AC-V66 vieA::pIVET5This work
 AC-V311AC-V66 vieS::pIVET5This work
 AC-V336AC-V66 ΔvieAB vieS::pIVET5This work
 AC-V279AC-V66 ΔvieABThis work
 AC-V282AC-V51 ΔvieSABThis work
 AC-V323AC-V51 ΔvieBThis work
 AC-V354AC-V66vieSA::pIVET5This work
Plasmids
 pIVET5oriR6K mobRP4 tnpR-lacZY, Apr5
 pCVD442oriR6K mobRP4 sacB, Apr7
 pAC301pIVET5::SfiI-Neor-SfiIThis work
 pAC298pIVET5::′vieA′This work
 pAC303pIVET5::vieS′This work
 pAC274pCVD442::ΔvieABThis work
 pAC275pCVD442::ΔvieSABThis work
 pSL108pCVD442::ΔvieBThis work
 pSL116pIVET5::vieSA′This work