Table 3.

Transcriptional assays of vieB

Growth medium and conditiona% TcsCFUb
LB broth0
M9 salts–0.2% (wt/vol) glucose–0.05% (wt/vol) CAA0
Low-iron LB broth0
Low-magnesium M9 salts–0.2% (wt/vol) glucose–0.05% CAA0
M9 salts lacking NH4 +–0.2% (wt/vol) glucose–0.05% CAA0
Syncase broth (microaerophilic)0
LB broth–0.2 or 0.4% (wt/vol) bile salts0
LB broth–1, 10, 20, or 50% (vol/vol) intestinal homogenate0
LB broth–5 mM glutathione0
Acid shock at pH 8, 7, 6, or 5.70
AKI (microaerophilic) (ToxR/ToxTON)0
CaCo-2 and HCT-8 intestinal cell lines0
Mouse intestinal infection80
  • a Growth conditions are described in Materials and Methods. CAA, Casamino Acids.

  • b The percent Tcs CFU was determined by replica plating colonies onto LB agar supplemented with 2 μg of tetracycline per ml and is a measure of the transcriptional activity of the vieB::tnpR-lacZYfusion.