Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaSource or reference
Strains
P. fluorescens2-79Phz+ wild type 36
P. fluorescens2-79RN10 Phz+ RifrNalr 36
P. fluorescens 2-79MXCPhz Rifr phzC::Kanr This study
P. fluorescens 2-79MXDPhzRifr phzD::Kanr This study
P. fluorescens 2-79MXEPhzRifr phzE::Kanr This study
P. fluorescens 2-79MXGPhz+Rifr phzG::Kanr This study
P. fluorescens 2-79.8APhzRifr phzE::Tn5 37
P. aureofaciens 30-84Phz+ wild type 25
E. coli JM109F′traD36 proA + proB+lacI q lacZΔM15/recA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB) mcrA Promega
E. coli HB101Δ(gpt-proA)62 leuB6 thi-1 lacY1 hsdS B 20 recA rpsL20 ara-14 galK2 xyl-5 mtl-1 supE44 mcrB B 1
E. coli S17-1 thi pro hsdR hsdM recA rpsL RP4-2 (Tetr::Mu) (Kanr::Tn7) 31
E. coli BL21F ompT hsdS B(rB mB ) gal dcm 35
E. coli BL21(DE3)F ompT hsdS B(rB mB ) gal dcmcIts857 ind1 Sam7nin5 lacUV5-T7gene1) 35
E. coliBL21(DE3)/pLysSF ompT hsdS B(rB mB ) gal dcmcIts857 ind1 Sam7nin5 lacUV5-T7gene1)pLysS 23
E. coli C2110K-12 gyrA polA1 his rha 34
Plasmids
 pBluescript II KS(SK)ColE1 f1(+/−) bla Stratagene
 pT7-5, pT7-6T7 promoter ColE1 bla 1
 pET-3aT7 promoter ColE1 bla 35
 pGP1-2Source of T7 RNA polymerase p15A 1
 pUC4KSource of Kanrcassette ColE1 bla Pharmacia
 pJQ200SKp15AaacC1 Mob+ sacB 29
 pHoHoTn3 delivery vectorlacIZYA+ tnpA bla 34
 pSSheHelper plasmid for Tn3 mutagenesis;tnpA cat 34
 pRK2013Helper plasmid; ColE1 Kanr Mob+Tra+ 9
 pPHZ108ApLAFR3 containing P. fluorescens 2-79 genomic DNA, Phz+ 37
 pT7-5FABCDpT7-5 containing 5.7-kb EcoRI-HindIII fragment withphzFABCD genes from P. aureofaciens 30-84This study
 pT7-5X-DpT7-5 containing 6.9-kbPstI-HindIII fragment withphzXYFABCD genes from P. aureofaciens30-84This study
 pT7-6A-GpT7-6 containing 6.9-kbBglII-XbaI fragment from pPHZ108A withphzABCDEFG genesThis study
 pT7-6ABCDpT7-6 containing 4.5-kb BglII-PstI fragment from pPHZ108A with phzABCD genesThis study
 pT7-6ABpT7-6 containing 1.3-kbBglII-EcoRV fragment from pPHZ108A withphzAB genesThis study
 pT7-6BpT7-6 containing 1.4-kb XhoI-EcoRV fragment from pPHZ108A withphzB geneThis study
 pT7-5CDpT7-5 containing 2.0-kb EcoRI-KpnI fragment from pPHZ108A withphzCD genesThis study
 pT7-5CDEpT7-5 containing 4.3-kb EcoRI-BglII fragment from pPHZ108A withphzCDE genesThis study
 pT7-6CDEFGpT7-6 containing 5.8-kb EcoRI-XbaI fragment from pPHZ108A with phzCDEFG genesThis study
 pT7-5GpT7-5 containing 1.5-kbBglII-XbaI fragment from pPHZ108A withphzG geneThis study
 pET-3XYpET-3a containingphzX and phzY genesThis study
  • a bla, β-lactamase; aaC1, gentamicin acetyltransferase-3-1; cat, chloramphenicol acetyltransferase; Kanr, kanamycin resistance; Rifr, rifampin resistance; Nalr, nalidixic acid resistance.