Table 1.

Bacterial strains and plasmids

Bacterial strain or plasmidRelevant characteristic(s)Reference
A. tumefaciens
 C58Wild-type virulent strain containing nopaline type Ti plasmid pTiC58 22
 NT1RERifampin- and erythromycin-resistant mutant of Ti plasmid-free strain A. tumefaciensC58 18
 NT1REBFlagellum-free mutant of NT1RE 6
Plasmids
 pJK270Tn5 insertion in the T-DNA of conjugative proficient pTiC58TraC 18
 pJK502Tn5 insertion in virB3 of pTiC58TraC 23
 pJK190Tn5 insertion in virB4 of pTiC58TraC 23
 pJK104Tn5 insertion in virB5 of pTiC58TraC 23
 pJK125Tn5 insertion in virB9 of pTiC58TraC 23
 pJK210Tn5 insertion in virB10 of pTiC58TraC 23
 pUCD4606 virB2 nonpolar mutant of pJK270 13
 pUCD2614High-copy-numbervir region plasmid derived from pTiC58 26