Table 2.

HCN biosynthesis in P. fluorescens CHA0 andE. coli BL21 harboring hcnABCrecombinant plasmids

StrainRelevant genotypeHCN productiona (μM)
CHA0 hcnABC+ 89 ± 5
CHA77 Δhcn <5
CHA77/pME3013 hcnABC+ 96 ± 32
CHA77/pME3206PKm -hcnABC+ 855 ± 90
BL21/pEB16<5
BL21/pME3210PT7 -hcnABC′ 132 ± 25
BL21/pME3212PT7 -hcnABC+ 102 ± 13
  • a P. fluorescens was grown in MMC with O2 limitation and E. coli was grown in a rich, glucose-containing medium as indicated in Materials and Methods. Mean ± standard deviation values for triplicate experiments are given.