Table 4.

Competition between the wild-type strain CHA0 and theanr mutant CHA21 (Kmr), with and without complementing plasmid pME3817, in YEA mediuma

Incubation (h)% Kmr colonies in mixtures of:
CHA0 and CHA21bCHA0 and CHA21/pME3817c
05659
15757
154745
243245
36446
48349
  • a Pairs of competing strains were set up by mixing overnight cultures 1:1 (by volume) and diluting the mixtures 50-fold with fresh YEA medium (60 ml) in 120-ml flasks. Incubation of the tightly closed flasks at 30°C resulted in oxygen-limited stationary-phase cultures (approximately 109 cells/ml) after 10 to 15 h.

  • b Mean values of triplicate experiments.

  • c Mean values of duplicate experiments.