Table 1.

Plasmids used in this study

PlasmidConstructionaStatus of pSAM2 derivatives in S. lividansb Reference
pOS546pOS548 derivative constructed with pTS74 (see Materials and Methods)Status after establishing: INT without tipAp induction; free and INT with tipAp inductionThis work
pOS548pTS39 derivative in which the pra gene promoter was replaced by tipAp (see Materials and Methods)Status after establishing: INT without tipApinduction; REP and INT with tipAp inductionThis work
pOS549pOS546 derivative with the pra gene disrupted by filling of the Asp718I(3302) siteNT in S. lividans; free and INT in S. lividans/pOS689This work
pOS550pOS548 derivative with the 3′ part of the pra gene deleted (2-kb deletion in thepra-traSA-spdA region)NT in S. lividans This work
pOS693pOS548 derivative with therepSA gene disrupted by insertion of Ωaac(3) in the ApaI(18514) siteNT inS. lividans This work
pOS11ΔDeletion variant of pOS11 (22); high copy number in S. lividans REP and INT 24
pTO1*TO1 derivative which does not contain the tipA promoter; integrative vector containing pBR322, a fragment of phage φC31 with its attP site and int gene for integration inStreptomyces spp. 28 This work
pOS689 BglII fragment from pOS541 (21) containing ermE*p-RBS-pra cloned in the BamHI site of pTO1*This work
  • a tipAp is the inducible tipApromoter, and ermE*p is the constitutive ermE*promoter. The numbers after some restriction sites correspond to the positions of the sites in Fig. 1. RBS, ribosome binding site.

  • b INT, integrative; REP, replicative; NT, no transformants.