Table 1.

A. tumefaciens strains and plasmids used in this study

Strain or plasmidaRelevant characteristicsAntibiotic resistancebSource or reference
Strains
 A136Nononcogenic strain; lacks pTiRifr 43
 A208Oncogenic nopaline-type strain; pTiT37Rifr 36
 A348Oncogenic octopine-type strain; pTiA6Rifr 14
 At221Tn3-HoHo1 insertion in virE2; A348mx358Carr Rifr 38
 At789A348(pBISN1)KanrRifr 31
 At793A136(pBISN1)KanrRifr 31
 At900LBA4404(pSa)Chlr GenrKanr Rifr SperSulr This study
 At901LBA4404(pSa::neo)ChlrGenr Kanr Neor RifrSper Sulr This study
 At902LBA4404(pUCD3960)Carr RifrSper This study
 At903LBA4404(pUCD5533)Carr RifrSper This study
 At904At221(pSa)Carr ChlrGenr Kanr Rifr SperSulr This study
 At905At221(pSa::neo)CarrChlr Genr Kanr NeorRifr Sper Chlr This study
 At906At221(pUCD3960)Carr RifrSper This study
 At907At221(pUCD5533)Carr RifrSper This study
 At915LBA4404(pToMoV-A)KanrRifr R. Gilbertson
 At916LBA4404(pToMoV-B)KanrRifr R. Gilbertson
 At925A208(pBISN1, pUCD5542, pSa)Chlr Genr KanrRifr Sper SulrTetr This study
 At926A208(pBISN1, pUCD5542, pUCD3960)Carr Kanr RifrSper Tetr This study
 At927A208(pBISN1, pUCD5542, pUCD5533)CarrKanr Rifr SperTetr This study
 At928A208(pBISN1)KanrRifr This study
 At929A208(pBISN1, pSa)Chlr Genr KanrRifr Sper Sulr This study
 At931A208(pBISN1, pUCD3960)CarrKanr Rifr Sper This study
 At932A208(pBISN1, pUCD5533)CarrKanr Rifr Sper This study
 At935A208(pBISN1, pUCD5542)KanrRifr Tetr This study
 At985At221(pJB31)Carr SperRifr This study
 At989At221(pBISN1)Carr KanrRifr This study
 At990At221(pBISN1, pUCD3960)Carr Kanr RifrSper This study
 At991At221(pBISN1, pUCD5533)Carr Kanr RifrSper This study
 At1042LBA4404(pToMoV-A, pSa)Chlr Genr KanrRifr Sper Sulr This study
 At1043LBA4404(pToMoV-B, pSa)ChlrGenr Kanr Rifr SperSulr This study
 At1044LBA4404(pToMoV-A, pSa::neo)Chlr GenrKanr Neor Rifr SperSulr This study
 At1045LBA4404(pToMoV-B, pSa::neo)Chlr GenrKanr Neor Rifr SperSulr This study
 At1046LBA4404(pToMoV-A, pUCD3960)Carr Kanr RifrSper This study
 At1047LBA4404(pToMoV-B, pUCD3960)Carr Kanr RifrSper This study
 At1048LBA4404(pToMoV-A, pUCD5533)Carr Kanr RifrSper This study
 At1049LBA4404(pToMoV-B, pUCD5533)Carr Kanr RifrSper This study
 At1050LBA4301(pToMoV-B, pJK703)Kanr Neor Rifr This study
 At1085A208(pJB31, pUCD5542)SperRifr Tetr This study
 LBA4301 rec mutant of A. tumefaciensAch5; no pTiRifr 24
 LBA4404Disarmed octopine-type strain; lacks T-DNARifr 33
Plasmids
 pBISN1T-DNA binary vector;nos-nptII and superpromoter-gusA intron geneKanr 31
 pJB31RSF1010 derivativeSper 1
 pJK703pTiC58virD::Tn5 Neor 35
 pSaOncogenic suppressive plasmidChlrGenr Kanr SperSulr 28
 pSa::neoNeomycin phosphotransferase gene disruption of the osa gene of pSaChlr GenrKanr Neor SperSulr This study
 pToMoV-ADimer of the A component of the ToMoV genome cloned into pCGN1547Kanr R. Gilbertson
 pToMoV-BDimer of the B component of the ToMoV genome cloned into pCGN1547Kanr R. Gilbertson
 pUCD105Stable cloning vector for A. tumefaciens; pTAR oriCarr KanrSper 41
 pUCD2001Low-copy cloning vector for A. tumefaciens CarrKanr Tetr 13
 pUCD3960 osa gene under the control of anpt promoter in pUCD105CarrSper 3
 pUCD5533 Asp718-blunted derivative of pUCD105Carr Sper This study
 pUCD5542 virB9, -10, and -11genes under the control of the virB promoter in a modified pUCD2001Tetr This study
  • a Laboratory stocks: A, E. W. Nester; At, S. B. Gelvin; LBA, R. A. Schilperoort; UCD, C. I. Kado.

  • b Car, carbenicillin; Chl, chloramphenicol; Gen, gentamicin; Kan, kanamycin; Neo, neomycin; Rif, rifampin; Spe, spectinomycin; Sul, sulfonamide; Tet, tetracycline.