Table 3.

Determination of Km values of cognate amino acid substrates and ATP of internal adenylation domains LicA1-A, LicB2-A, and LicC-A

Tested domainSubstrateKm(mmol · liter−1) ± SD
Amino acidATPa
LicA1-A l-Gln1.4 ± 0.55 ± 3
LicB2-A l-Asp0.3 ± 0.151 ± 0.3
LicC-A l-Ile0.4 ± 0.150.1 ± 0.02
LicC-A l-Leu3 ± 15 ± 2
LicC-A l-Val2.5 ± 10.6 ± 0.2
  • a Experiments were performed in the presence of the specific substrate amino acid.