Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsSource or reference
Strains
S. parasanguis
  FW213Wild type 6
  VT1354FW213recA::aphA-3; Kmr This study
E. coli JM109Host strain for cloning 32; ATCCa
Plasmids
 pT7BluePCR cloning vector; Apr Novagen
 pVT1185640-bp PCR-derivedrecA fragment ligated into “T” cloning site of pT7 Blue; Apr This study
 pVT1148pVT1185 with kanamycin resistance gene inserted at EcoRV site of recAfragment; Apr Kmr This study
 pVA981Replicates in E. coli but not in streptococci; Tcr 26
 pVT12902.2-kb insert from pVT1148 containing disruptedrecA fragment ligated into pVA981; KmrTcr This study
 pVT13563.1-kbHindIII-EcoRI fragment containing entirerecA gene ligated intoHindIII-EcoRI sites of pT7Blue; Apr This study
  • a ATCC, American Type Culture Collection.