Table 3.

Effects of thiol reagents and metal ions on PDC hydrolase activitya

ReagentConcn (mM)Relative activity (%)
PDC hydrolysisPDC synthesis
Ellman’s reagent0.11020
N -Ethylmaleimide156
Iodoacetic acid19090
MnCl2 · 4H2O0.2100100
CoCl2 · 6H2O0.2100100
CuSO4 · 5H2O0.210040
ZnSO4 · 7H2O0.2510
  • a Purified LigI (0.5 μg) was preincubated with each reagent at 30°C for 10 min, and the remaining activities were determined.