Table 1.

Bacterial strains, plasmids, and phages

Strain, plasmid, or phageGenotype or relevant phenotypeSource or reference
S. peucetius ATCC 29050Wild typeAmerican Type Culture Collection
 WMH1530dnrN::aphII46
 WMH1650dnrX::aphII40
 WMH1658dnrU::aphIIThis work
 WMH1662dnrX::aphII dnrU::aphIIThis work
 WMH1663dnrV::aphIIThis work
 WMH1664, WMH1665doxA::aphIIThis work
 WMH1666dpsH::aphIIThis work
 WMH1667, WMH1668dnrX::aphII dnrU::aphII dnrH::aac(IV); SLP1, SLP2, spectinomycin resistantThis work
S. lividansTK2330
 TK24SLP1, SLP2, streptomycin resistant30
 WMH1594SLP1, SLP2, streptomycin resistant, φC31 lysogen40
Plasmids
 pWHM249XhoI site eliminated from theaphII gene in pFDNeoS40
 pWHM5556.0-kb BamHI fragment cloned from pWHM335 into pUC19This work
 pWHM2894.8-kbBamHI-NruI fragment cloned from pWHM555 into pSE380This work
 pWHM2904.8-kbBamHI-XhoI fragment cloned from pWHM299 into pSP72This work
 pWHM2931.0-kb SalI fragment containing aphII gene cloned blunt ended from pWHM249 into the filled-in BalI site of pWHM290This work
 pWHM2944.0-kb AatII-XhoI fragment containing the aphII gene cloned blunt ended from pWHM293 into the filled-in PvuII site of pSP72This work
 pWHM2981.55-kb AatII-SstI fragment cloned from pWHM555 into pSE380This work
 pWHM2991.55-kbEcoRI-HindIII fragment containing thednrU gene cloned from pWHM298 into pWHM3This work
 pWHM3733.1-kb PvuII-SstI fragment cloned from pWHM555 into pSP72This work
 pWHM3743.1-kbEcoRI-XhoI fragment cloned from pWHM373 into pSE380This work
 pWHM3753.1-kbEcoRI-HindIII fragment containingdnrU and dnrV genes cloned from pWHM374 into pWHM3This work
 pWHM3811.0-kb SalI fragment containing the aphII gene cloned blunt ended from pWHM249 into the filled-in AatII site of pWHM374This work
 pWHM3824.1-kb EcoRI-XhoI fragment cloned from pWHM381 into pCEM7zf(+)This work
 pWHM3841.0-kb SalI-NotI fragment cloned from pWHM373 into pSE380This work
 pWHM3851.0-kbEcoRI-HindIII fragment containing thednrV gene cloned from pWHM384 into pWHM3This work
 pWHM3862.3-kb BamHI-NotI fragment cloned from pWHM290 into pSE380This work
 pWHM3872.3-kb EcoRI-HindIII fragment containing the dnrV and doxA genes cloned from pWHM386 into pWHM3This work
 pWHM3881.6-kbAgeI-BamHI fragment cloned from pWHM555 intoXmaI-BamHI sites of pCMV SPORT3This work
 pWHM3891.6-kb EcoRI-HindIII fragment cloned from pWHM388 into Litmus 38This work
 pWHM3901.6-kb EcoRI-HindIII fragment containing the doxA gene cloned from pWHM389 into pWHM3This work
 pWHM3911.0-kb SalI fragment containing aphII gene cloned blunt ended from pWHM249 into the filled-in BspEI site of pWHM555This work
 pWHM3924.4-kb BamHI-SstI fragment cloned from pWHM391 into pSE380This work
 pWHM3941.0-kbSalI fragment containing the aphII gene cloned blunt ended from pWHM249 into the filled-in NcoI site of pWHM290This work
 pWHM3953.5-kbNotI-XhoI fragment cloned from pWHM394 into pSE380This work
 pWHM5463.7-kb SstI fragment containing the dnrU, dnrV, and doxAgenes cloned into pWHM3This work
 pWHM5472.3-kbNotI-BamHI fragment containing thednrV and doxA genes cloned under the control of the ermEp* promoter in pWHM3This work
 pWHM5481.4-kb KpnI-BamHI fragment containing the doxA gene cloned under the control of the strong constitutive ermEp* promoter into pWHM3This work
 pWHM9590.4-kb internal segment of the dnrH gene cloned into the temperature-sensitive pKC1139 plasmid carrying theaac(IV) apramycin resistance gene52
Phages
 KC515c+ attP, thiostrepton resistant, viomycin resistant11
 phWHM295c+attP::vph::4.0-kbXhoI-BamHI fragment::aphII; 4.0-kb XhoI-BamHI fragment cloned from pWHM294 into KC515This work
 phWHM353c+attP::vph:4.1-kb XhoI-BamHI fragment::aphII; 4.1-kbXhoI-BamHI fragment cloned from pWHM352 into KC515This work
 phWHM363c+attP::vph::4.4-kbXhoI-BamHI fragment::aphII; 4.4-kb XhoI-BamHI fragment cloned from pWHM362 into KC515This work
 phWHM366c+attP::vph::3.5-kbXhoI-BamHI fragment::aphII; 3.5-kb XhoI-BamHI fragment cloned from pWHM365 into KC515This work