Table 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
KC2668hutC515Δ[bla]-2 nac+10
KC2725hutC515 Δ[bla]-2 nac-203::Tn5-13110
KC2972KC2941/pCB511Transformation
KC3220KC2725/pCB594Transformation
KC3555KC2725/pCB771Transformation
KC3568KC2725/pCB774Transformation
KC3572KC2725/pCB778Transformation
KC3578KC2725/pCB781Transformation
KC3580KC2725/pCB782Transformation
KC3591KC2725/pCB788Transformation
KC3615KC2725/pCB800/pCB648Transformation
KC3973KC2725/pCB941Transformation
KC3977KC2725/pCB529Transformation
KC3981KC2725/pCB606Transformation
KC4510KC2725/pCB547Transformation
Plasmids
pCB511pGB2; wild-typenacEaThis laboratory
pCB529pGB2; nac-10 allele of E. coliThis laboratory
pCB547pGB2; nac-28allele of E. coliThis laboratory
pCB594pMalC2; MBP-NACE fusion proteinThis laboratory
pCB606pMalC2; MBP-NACK fusion proteinThis laboratory
pCB771pGD103; NACE-his6This laboratory
pCB774pGD103; wild-type NACEThis laboratory
pCB778pGD103; wild-type NACKThis laboratory
pCB781pGD103; NACK-his6This laboratory
pCB782pGD103; NACK-(DFGRSGHTDSL)This laboratory
pCB788pGD103; NACK100-his6This laboratory
pCB800pGD103; NACK120-his6This laboratory
pCB941pGD103; NACE100-his6This laboratory
pGB2Low-copy cloning vector; insert expressed from its own promoter4
pGD103Low-copy vector expressing inserted material fromlacP5
pMalC2MBP fusion vector expressing inserted material from tac promoterNew England BioLabs
  • a Vector; cloned insert or product.