Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
Strains
E. coli
  DH5α supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 56
  SM10Mobilizing strain; RP4 tra genes integrated in chromosome; Kmr 62
  MG4Δ(argF-lac)U169 zah-735::Tn10 recA56 srl::Tn10 53
  HB101 supE44 hsdS20(rBmB) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 56
  VJS533 recΔA56 ara Δ(lac-proAB)X111 rpsLφ80dΔ(lacZ)M15 70
B. cepacia
  K56-2Cystic fibrosis respiratory isolate 9
  K56-R2 cepR::Tn5-OT182 derivative of K56-2This study
  K56-I2 cepI::tmp derivative of K56-2This study
R. solanacearum AW1-A18 solI8::SP 19
Plasmids
 pOT182pSUP102(GM)::Tn5-OT182 Cmr Gmr AprTcr 38
 pNOT19Modified pUC19 cloning vector; Apr 57
 pUCP28TBroad-host-range vector; IncP OriT pRO1600 ori Tpr 58
 p34E-TpSource oftmp cassette; Tpr 11
 pRK2013ColE1 Tra (RK2)+Kmr 18
 pEX18TcSuicide vector; sacB Tcr 26
 pCRR2.1TOPOCloning vector for PCR productsInvitrogen
 pSLR2-111.3-kb ClaI fragment from K56-R2 obtained by self-cloning; Tcr This study
 pSLR2-211.5-kb XhoI fragment from K56-R2 obtained by self-cloning; Tcr This study
 pSLA3.2pUCP28T with 3.2-kb SphI fragment from K56-2 containing cepIR genes; Tpr This study
 pSLR100pUCP28T with 1.65-kb KpnI-SphI fragment from pSLA3.2 containing cepR gene; Tpr This study
 pSLS201pNOT19 with 1.55-kbSphI-KpnI fragment from pSLA3.2 containingcepI gene; Apr This study
 pSLS201-TpNOT19 with 2.2-kb fragment containingcepI gene inactivated with tmp cassette; Apr Tpr This study
 pEXCEPIPCR product (inactivated cepI fragment from pSLS201-T) in pEX18Tc; Tcr Tpr This study
 pKDT17 lasB::lacZ plac-lasRApr 48
 pHV2001 luxR luxI′CDABEApr 49
 p395B aidA::lacZTcr 19
 pECP61.5 rhlR rhlA::lacZApr 51