Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotypeReference or source
S. flexneri strains
 2457TWild-type serotype 2a 10
 BS1092457TgalU1::Tn10 34
 SS1002457T ΔgalU2 This work
 MBG2832457T icsA1 38
 SSW201SS100cydC1::Tn10 This work
 SSW301SS100pepP1::Tn10 This work
 SSW401SS100ribE1::Tn10 This work
 SSW2022457TcydC1::Tn10 This work
 SSW3022457TpepP1::Tn10 This work
 SSW4022457TribE1::Tn10 This work
 SSW203MBG283cydC1::Tn10 This work
 SSW303MBG283pepP1::Tn10 This work
 SSW403MBG283ribE1::Tn10 This work
E. coli DH10BF mcrAΔ(mrr-hsdRMS-mcrBC) f80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu)7697 galU galK λ rpsL nupG GibcoBRL
Plasmids
 LITMUS38Cloning vector: Amr New England Biolabs
 pSSW204LITMUS38 containing intact S. flexneri cydDC; Amr This work
 pMBG462pSSW204 with two frame-shift mutations introduced into cydD, such thatcydC is in the native reading frameThis work