Table 1.

Bacterial strains and plasmids used

Strain or plasmidRelevant characteristicsSource or reference
P. aeruginosa strains
 PAO1Wild typeThis laboratory
 PAO-JP2ΔlasI ΔrhlITcr HgCl2 r 42
 PAO-JP3ΔlasR rhlR::Tn501Tcr HgCl2 r 42
 PAO200Δ(mexA-mexB-oprM) unmarked 53
E. coliMG4λI14Δ(argF-lac)U169zah-735::Tn10 recA56 srl::Tn10λ::lasIp-lacZ 54
Plasmids
 pPCS1ColE1 lasR + bla 54
 pECP61.5ColE1oriP. aeruginosa rhlAp-lacZ tacp-rhlR + bla 42
 pUCP21T oriP. aeruginosabla 53
 pPS952pUCP21T derivative;mexA + mexB + oprM + 53