Table 1.

Phenotypes of strains NH1 and NH2

StrainAmt (μg/ml) of virginiamycin producedaVB binding activ- ityb (pmol/mg of protein)Amt (nM) of VB producedc
Wild type860.44390
NH11000
pGM122-integrated 00.14NT
NH200364
  • a Virginiamycin production was determined after 24 h of cultivation.

  • b VB binding activity was determined after 12 h of cultivation.

  • c VB production was tested after 24 h of cultivation. NT, not tested.