Table 1.

Bacteria and plasmids used

Strain or plasmidRelevant characteristicsaReference and/ or source
P. putida
 KT2440 hsdMR 10
 KT2442 P. putida KT2440 Rifr V. de Lorenzo
 R6C1Smr SucsLux+; P. putida KT2440 cointegrate containing pMIR592This study
 C1R1Lux+; RpoS derivative from KT2440This study
E. coli
 ZK918W3110 ΔlacU169 tna-2 λMAV103rpoS::kan 3; G. W. Huisman
 HB101FΔ(gpt-proA)62 leuB6 supE44 ara-14 galK2 lacY1Δ(mcrC-mrr) rpsL20 (Smr)xyl-5 mtl-1 recA13 4
 DH5αF′F′/endA1 hsdR17(rK mK +) supE44 thi-1 recA1 gyrA (Nalr) relA1Δ(lacIZYA-argF)U169 deoR[φ80dlacΔ(lacZ)M15] 48
 CC118(λpir)λpirRifr 16
Plasmids
 pRK600Cmr; mob tra V. de Lorenzo
 pLAFR3Tcr derivative from the cosmid pLAFR1 (11) modified to include multiple cloning sites and the Plac promoter fused to the α peptide of ′LacZJ. L. Ramos
 pUTSmSmrApr; mini-Tn5/′Sm element inserted in pUT 16
 pUC18 and pUC19Apr, multiple cloning site, Plac promoter fused to the α peptide of ′LacZ 46
 pUNØ18 and pUNØ19Apr; identical to pUC18 and pUC19 except that the NheI site was changed to a NotI site andoriT was inserted as a 0.7-kb fragment in the newNotI siteSilvia Marqués
 pUNØ19AApr; pUNØ19 after removing its uniqueAatII siteThis study
 pUJ20TcrApr; mini-Tn5/′luxABTc element inserted in pUTV. de Lorenzo
 pMIR0-1Tcr; chimeric cosmid of P. putida library bearing a gene homologous to therpoS gene of E. coli This study
 pMIR1-2Tcr; chimeric cosmid of P. putida library bearing a gene homologous to the rpoSgene of E. coli This study
 pMIR1-34Tcr; chimeric cosmid of P. putida library bearing a gene homologous to the rpoSgene of E. coli This study
 pMIR2-9Tcr; chimeric cosmid of P. putida library bearing a gene homologous to the rpoSgene of E. coli This study
 pMIR13415Tcr Smr; mini-Tn5/′Sm element inserted in the P. putida rpoS gene on the 3.4-kb EcoRI fragment of pMIR1-34This study
 pMIR13450Apr; 3.4-kbEcoRI fragment of pMIR1-34 was inserted in pUN19ØThis study
 pMIR11Apr; 7-kb BamHI fragment of pMIR2-9 was inserted in pUN19ØThis study
 pMIR61Apr; rpoS gene as a 2.5-kbBamHI/NheI P. putida DNA fragment inserted at the BamHI and XbaI sites of pUN19ØThis study
 pKNG101 strAB mobRK2 oriR6K sacBR 19
 pMIR492Apr;luxAB cassette as a SalI/BamHI fragment from pUJ20 inserted at AatII unique site of pMIR61This study
 pMIR592Smr; PrpoS::luxAB fusion asKpnI/SphI fragment from pMIR492 inserted at theSmaI site of pKNG101This study
 pGM112Kmr Cmr bolAp 1::lacZ 26
 pGM115Kmr Cmr ficp::lacZ 26
 pGM118Kmr Cmr lacZpromoterless 26
  • a Ap, ampicillin; Cm, chloramphenicol; Km, kanamycin; Lux, light emission; Nal, nalidixic acid; Rif, rifampin; Sm, streptomycin; Suc, sucrose; Tc, tetracycline.