Table 2.

Functional characterization of plasmids expressing SOS functions

ExptE. coli strains (relevant genotype) [plasmid-expressed SOS genes]UV (J/m2)IPTG (1 mM)Survival (%)No. of rifampin-resistant mutants/ 106 survivors
AKH201000.06
KH25021.411.4
BSR100 (ΔumuDC)01000.14
SR100 (ΔumuDC)507.40.58
CSR230 (ΔumuDC) [pSE380 vector]0+1000.04
SR230 (ΔumuDC) [pSE380 vector]50+9.50.97
DSR420 (ΔumuDC) [Ptrc-umuD′C]01000.03
SR420 (ΔumuDC) [Ptrc-umuD′C]509.61
ESR420 (ΔumuDC) [Ptrc-umuD′C]0+1000.16
SR420 (ΔumuDC) [Ptrc-umuD′C]50+18.115
FSR400 (ΔumuDC ΔrecA)01000.09
SR400 (ΔumuDC ΔrecA)50.0060.005
GSR440 (ΔumuDC ΔrecA) [Ptrc-umuD′C recA]01000.14
SR440 (ΔumuDC ΔrecA) [Ptrc-umuD′C recA]500.042.7
HSR440 (ΔumuDC ΔrecA) [Ptrc-umuD′C recA]0+1000.48
SR440 (ΔumuDC ΔrecA) [Ptrc-umuD′C recA]50+1514.3