Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotypeReference or source
Strains
 CY15076W3110tnaA2 ΔlacU169This study
 PDG1158SVS1100 (λtnaptnaC-UAA-tnaA′-′lacZ)17
 PDG1184SVS1100 (λtnaptnaC-UUA-tnaA′-′lacZ)17
 SVS1100W3110 bglR55Δ(lac-argF)U16946
 SVS1144SVS1100 (λtnaptnaA′-′lacZ)46
 VK100SVS1100 (λtnaptnaC-UGA-′lacZ)28
 VK100/SerUUGAVK100 serU [Ser UG(A/G)] suppressor28
 VK700CY15076 (λtnap tnaC-UAG-′lacZ)This study
 VK700/SerUUAGVK700 serU (Ser UAG suppressor)This study
 VK701VK700prfA1(Ts)This study
 VK800CY15076 (λtnaptnaC-UAG-tnaA′-′lacZ)This study
 VK801VK800 prfA1(Ts)This study
 VK805VK801 trpA9761This study
 VK900CY15076 (λtnaptnaC-UAA-′lacZ)This study
 VK1100CY15076 (λtnap tnaC-UAG UUUGAC-tnaA′-′lacZ)This study
 VK1101VK1100prfA1(Ts)This study
 VK1145SVS1144prfA1(Ts)This study
 VK1159PDG1158prfA1(Ts)This study
 VK1185PDG1184prfA1(Ts)This study
 VK1200CY15076 (λtnap tnaC-UAG UUGACC-tnaA′-′lacZ)This study
 VK1201VK1200prfA1(Ts)This study
Plasmids
 pGFIBIhisT su (UAG suppressor)35
 pMY228trpT su7 (UAG suppressor)36
 pRS552pBR322 derivative, lac-based vector44
 pSWC115trpT su7(UAA suppressor)36