Table 1.

Plasmids and bacterial strains used

Plasmid or strainRelevant genotypeSource (reference)
Plasmids
 pSE380Vector (Ampr)R. Maurer (2)
 pSR1000pSE380 derivative expressing umuD′ and umuC genes under the control of a Ptrc promoterThis work
 pSR1007pSR1000 derivative expressing umuD′ andumuC genes under the control of a PtrcpromoterThis work
 pSR1718pSR1007 derivative expressingumuD′, umuC, and recA genes under the control of a Ptrc promoterThis work
 pEC42pBR322-derived plasmid bearing umuD′ andumuC genes under the control of a lambda pL promoterR. Woodgate (3)
E. coli
 KH2Sup0Δlac-pro trpE9777 (F′lacIqZΔM15pro+)This lab (21)
 KH2RSup0 Δlac-pro trpE9777Δ(srlR-recA)306::Tn10(Tetr) (F′ lacIqZΔM15pro+)This lab (16)
 GY8347FΔ(umuD/C)595::catS. Sommer (25)
 SR100Δ(umuD/C)595::catin KH2This work
 SR400Δ(srlR-recA)306::Tn10(Tetr) in SR100This work
 SR230SR100 bearing plasmid pSE380This work
 SR420SR100 bearing plasmid pSR1007This work
 SR440SR400 bearing plasmid pSR1718This work