Table 2.

Primers used in this study

PrimerSequenceaRestriction enzymea
OL585′ AAAAAAAACATATGAAGGACAGCACCATGTCTAC 3′ NdeI
OL595′ AAAAACTCGAGAAAAAGAGGAGTAGTGCCTG 3′ XhoI
OL725′ AAAAAAACTCGAGAGCGGTGTTTCTTCTGCGTG 3′ XhoI
OL745′ AAAAAAACTCGAGTTGTGCTGACACTTGTCGTG 3′ XhoI
OL755′ AAAAAAATCGATCCGAATACGATGGTCAATTAGATAG 3′ ClaI
OL765′ AAAAAAACTCGAGAACGGCAGTACATTTGAGTGG 3′ XhoI
OL775′ AAAAAAATCGATCCTCCAACTAAAGCTAATCCTAGTG 3′ ClaI
OL915′ AAAAAAAAACTCGAGCTGATCAGCTTCATTGCT 3′ XhoI
OL935′ AAAAAAACTCGAGTTTATCCTCGCTATGCGC 3′ XhoI
OL945′ AAAAAAAAACATATGGCATTTAACGACATTAAA 3′ NdeI
OL995′ AAAAACTCGAGCATATGTTTCGATTGTTTCAC 3′ NdeI
  • a The restriction endonuclease site in each oligonucleotide is underlined, and the enzyme that cuts it is listed at the right.