Table 4.

Effects of SOS and UVM pathways on base insertion opposite an AP site

E. coli strain (relevant genotype) [plasmid-expressed SOS genes]InductionaAvg survivalb (IC/ml) ± SDBase insertion opposite AP site (avg % ± SD)c
SOSUVMAP:A (AP→T)AP:T (AP→A)AP:G/C (AP→C/G)AP:0 (AP→−1)
SR420 (ΔumuDC) [Ptrc-umuD′C]405 ± 20516.6 ± 3.91.2 ± 0.28.4 ± 5.373.8 ± 2.3
SR420 (ΔumuDC) [Ptrc-umuD′C]+470 ± 21239.5 ± 9.12.9 ± 1.36.9 ± 6.950.7 ± 2.4
SR420 (ΔumuDC) [Ptrc-umuD′C]+1,805 ± 75762.9 ± 6.33.7 ± 2.712.4 ± 2.521.1 ± 5.1
SR420 (ΔumuDC) [Ptrc-umuD′C]++2,885 ± 1,97364.3 ± 7.56.1 ± 1.422.4 ± 3.37.2 ± 2.9
SR440 (ΔumuDC ΔrecA) [Ptrc-umuD′C recA]520 ± 32549.6 ± 3.31.3 ± 0.47.7 ± 2.841.3 ± 0.9
SR440 (ΔumuDC ΔrecA) [Ptrc-umuD′C recA]+700 ± 45343.4 ± 2.46.8 ± 0.47.3 ± 2.642.5 ± 0.8
SR440 (ΔumuDC ΔrecA) [Ptrc-umuD′C recA]+1,643 ± 74276.6 ± 5.93.3 ± 1.214.2 ± 6.35.9 ± 0.8
SR440 (ΔumuDC ΔrecA) [Ptrc-umuD′C recA]++2,910 ± 1,43972.6 ± 8.24.4 ± 0.816.2 ± 5.36.9 ± 4.1
SR400 (ΔumuDC ΔrecA)115 ± 352.4 ± 2.41.5 ± 0.63.1 ± 493 ± 6.4
SR400 (ΔumuDC ΔrecA)+100 ± 718.2 ± 2.71.8 ± 0.77 ± 2.683 ± 2.1
  • a Expression of the SOS genes is fully turned on (+) in strains SR440 and SR420 by including IPTG (1 mM) in the culture medium. UVM is induced (+) by exposing mid-log-phase cells to MNNG (10 μg/ml) at 37°C for 10 min followed by cell pelleting and washing.

  • b Measured as transfection efficiency. Data shown are averages of two to three independent transfections.

  • c Data shown are averages from at least two independent transfections (the same as the ones used for determining survival values) and six or more elongation reactions.