Table 1.

Purification of B. subtilis SodA

FractionProtein concn (mg/ml)Total protein (mg)Sp act (U/mg)Total UFold purificationEnzyme yield (%)
Crude extract5.7035814.75,2601100
Ammonium sulfate (70–90% saturated)3.008.101961,59013.330.2
DEAE Sepharose CL-6B0.1281.021,1201,15076.221.9
Mono Q0.1120.1522,5803921767.5