Table 1.

B. subtilis strains used in this study

StrainGenotypeSource or derivation and referencea
LMB1 trpC2 E. Ferrari, I168b
LMB10 trpC2 sigD::pLM5 (Cmr)M. J. Chamberlin, CB100b; 13
LMB24 trpC2 phe hag::lacZ (Cmr)M. J. Chamberlin, CB25b; 32
LMB213 trpC2 flgM::mini-Tn10(Spr)Transform [LMB1:HB4229, Spr]; reference 6 and this study
LMB214 trpC2 fla/chePAΔTransform [LMB1:pWE4-int, Cmr]c; this study
LMB216 trpC2 fla/che PAΔflgM::mini-Tn10(Spr)Transform [LMB214:LMB213, Spr]; this study
LMB219 trpC2 hag::lacZ(Cmr)Transform [LMB1:LMB24, Cmr]; this study
LMB220 trpC2 fla/che PAΔhag::lacZ(Cmr)Transform [LMB214:LMB24, Cmr]; this study
LMB221 trpC2 flgM::mini-Tn10 (Spr)hag::lacZ(Cmr)Transform [LMB219:LMB213, Spr]; this study
LMB222 trpC2 flgM::mini-Tn10 (Spr)fla/che PAΔhag::lacZ(Cmr)Transform [LMB220:LMB213, Spr]; this study
  • a Transformation of [first (recipient) strain: with chromosomal DNA from second strain, and selecting for resistance indicated] is shown for some strains.

  • b Previous designation of strain.

  • c This strain was subsequently cured of the chloramphenicol resistance marker.