Table 1.

PCR primers used in this study

PrimerSequence (5′→3′)
LmsigB-1AC(A/G) TGC ATT TGA (C/G)(A/T)T A(A/T)A CCG A
LmsigB-2G(C/T)G T(A/T)C ATG T(G/T)C CGA GAC G
LmsigB-3TTT GCG GCG AGC TTT GTA AT
LmsigB-4CAT CGG TGT CAC GGA AGA AGA A
LmsigB-10CCC GTT TCT TTT TGA CTG C
LmsigB-12CAT TTA TTG AAA ATC GCA GTC
LmsigB-15AAT ATA TTA ATG AAA AGC AGG TGG AG
LmsigB-16ATA AAT TAT TTG ATT CAA CTG CCT T
LmsigB-17ACT TGA TAT ACT TCA AGT GCT
LmsigB-18GGG TAT AGT ACT TCG AAT CG
LmsigB-19CGA ATA TTA CCC ATA CCA G
LmsigB-20TAT GAT TGG GAT GGA AG
LmrsbW-1AAG GG(A/T) GAC AG(C/T) TTT GA(C/T) T(A/T)T GA
LmrsbW-3GGC ATT CTC CTC CAC CTG
LmrsbW-4CTC AAC CTG ATA AAG AGG CG
SOE-Aa GGA ATT CCA GGT GGA GGA GAA TGC
SOE-Bb CAC ACG TTC AAA TCC ATC ATC TGC AGC AAA TGC TTC AAA G
SOE-CGAT GAT GGA TTT GAA CGT GTG
SOE-Dc GCT CTA GAA CAT CCC CGC AGT ATT G
  • a The EcoRI restriction site incorporated into this primer to facilitate cloning is underlined.

  • b The overhang complementary to SOE-C is underlined.

  • c The XbaI restriction site incorporated into this primer to facilitate cloning is underlined.