Table 1.

Strains of E. coli K-12 and plasmids used in this study

Strain or plasmidGenotypeSource or reference
Strains
 H1673W3110fur-31 zbf::Tn10 Klaus Hantke, Tübingen
 H1941MC4100 Δfur Klaus Hantke, Tübingen
 JM101Δ(lac-proAB)thi supE F′[traD36 proAB + lacI q lacZΔM15] 39
 JRG2712MC4100 ftnA::spc This work
 JRG2157JM101 bfr::kan, F This work
 JRG2844MC4100ftnA::spc This work
 JRG2846MC4100bfr::kan This work
 JRG2847JRG2846ftnA::spc This work
 JRG2951W3110bfr::kan This work
 JRG2952W3110ftnA::spc This work
 JRG2953JRG2951ftnA::spc This work
 JRG3236H1941bfr::kan This work
 JRG3238JRG3236ftnA::spc This work
 JRG3240H1941ftnA::spc This work
 MC4100Δara, Δ(argF-lac) rpsL relA1 deoC1 ptsF25 52
 W3110Prototroph
Plasmids
 pGS280 bfr amp 2
 pGS327 amp This work
 pGS357 kan amp This work
 pGS358 bfr::kan cml rep(Ts)This work
 pGS635 spc amp This work
 pGS636 ftnA::spc amp This work
 pGS637 ftnA::spc cml rep(Ts)This work
 pMAK705 rep(Ts) lacZ cml 27
 pMTL24 amp 13
 pUC18A2 ftnA amp 34
 pUX-Ω spc 44