Table 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
E. coliINVαF′ recA1 lacZΔM15Invitrogen
A. pernixK1Natural isolate of A. pernixJCM9820 50
Plasmids
 pGEM-3Zf(+)Cloning vector; Ampr Promega
 pCR2.1Cloning vector; AmprKmr Invitrogen
 pRU3634.5-kbBamHI-EcoRI chromosomal DNA fragment containing 5′ portion of arnS and its upstream region (nt 1–4477) inserted into pGEM-3Zf(+)This study
 pRD5426.2-kbEcoRI-SalI chromosomal DNA fragment containing 3′ portion of arnS and entire arnL (nt 4472–10717) inserted into pGEM-3Zf(+)This study
 pNB5348-bp PCR fragment containing the partial internal region of Iα (nt 4515–4862) inserted into pCR2.1This study
 pNC2663-bp PCR fragment containing the partial internal region of ES1 (nt 3380–4042) inserted into pCR2.1This study
 pIP23145-bp PCR fragment containing the partial internal region of Iβ (nt 7278–7322) inserted into pCR2.1This study
 pIP232228-bp PCR fragment containing the partial internal region of Iγ (nt 8439–8666) inserted into pCR2.1This study
 pNA41,444-bp reverse transcriptase-mediated PCR fragment containing entire cDNA of mature 16S rRNA inserted into pCR2.1 50
 pND31462-bp reverse transcriptase-mediated PCR fragment containing partial cDNA of mature 23S rRNA (nt 8913–9374) inserted into pCR2.1This study