Table 1.

Strains and plasmids used in this study

Strain or plasmidGenotype or phenotypeReference or source
Strains
 MC4100F araD139 Δ(argF-lac)U169 ptsF25 deoC1 relA1 flbB530 rpsL150λ 5
 FM420Like MC4100 butrecA56 43
 RM202Like MC4100 but Δpfl-25 Ω(pfl::catpACYC184) Δ(srl-recA)306::Tn10 32
 RM221Like MC4100 but Δ(pfl-act) Δ(srl-recA)306::Tn10 11
 RM226Like MC4100 but Δpfl-25Ω(pfl::cat pACYC184) ΔtdcE 11
 234M11Like MC4100 but Δact Ω(act::catpACYC184) Δ(srl-recA)306::Tn10 31
 JM109Δ(lac-proAB) recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 42
 W3110F IN(rrnD-rrnE)1 rph-1 λ 18
 W3110pfl Like W3110 but Δpfl-25Ω(pfl::cat pACYC184)This work
 K38 phoA tonA ampF 22
C. butyricum DSM-552Type strainGerman Culture Collection, Braunschweig, Germany
Plasmids
 pBR322TetrAmpr 4
 pT7-4Ampr, T7Φ10 34
 pT7-5Ampr, T7Φ10 34
 pT7-6Ampr, T7Φ10 34
 pGP1-2Kmr, T7 gene 1 (RNA polymerase) 34
 pMU10Like pBR322,tcbA′ tcbB+ tcbC+ This work
 pMU1011Like pBR322, tcbC + This work
 pMU1012Like pMU1011 but mutated in tcbC This work
 pMU1013Like pMU1011 but mutated intcbC This work
 pT7-1011Like pT7-5 buttcbC + This work
 pT7-1014Like pT7-1011 but with insert in the opposite orientation relative to the T7Φ10 promoterThis work